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Merck
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文件

N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

同義詞:

pNPP tablets

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About This Item

MDL號碼:
MFCD00284586
分類程式碼代碼:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ 对硝基苯基磷酸酯片, tablet, To prepare 5 mL

品質等級

200

形狀

tablet

溶解度

deionized water: soluble 5 mL

儲存溫度

−20°C

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相關類別

應用

SIGMA FAST p-磷酸硝基苯酯片剂用于：
  • 定量非分泌型碱性磷酸酶，监测成骨样细胞的分化情况（SaoS-2）
  • 染色小鼠前成骨细胞 MC3T3-E1，进行碱性磷酸酶测定
  • 用作 ExtrAvidin 碱性磷酸酶的酶作用物®，通过酶联免疫吸附法（ELISA）测定血红蛋白结合α-突触核蛋白
  • 用作碱性磷酸酶偶联抗体的酶作用物，进行Abirin毒素的ELISA检测
开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP，0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取，或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水，即可制备活性底物溶液。片剂单独包装在铝箔包装中 。

重構

将一组片剂溶于5 mL去离子水中，即可得到含有pNPP的即用型缓冲液。

法律資訊

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
Nontyphoidal Salmonella (NTS) organisms are a major cause of gastroenteritis and bacteremia, but little is known about maternally acquired immunity and natural exposure in infant populations residing in areas where NTS disease is highly endemic. We recruited 503 pregnant mothers
Lan Xu et al.
Experimental cell research, 360(2), 413-420 (2017-09-30)
The highly glycosylated bone sialoprotein (BSP) is an abundant non-collagenous phosphoprotein in bone which enhances osteoblast differentiation and new bone deposition in vitro and in vivo. However, the structural details of its different glycosylation linkages have not been well studied
Yuankun Zhai et al.
Journal of cellular physiology, 234(10), 18602-18614 (2019-03-27)
Homocysteine (Hcy) is detrimental to bone health in a mouse model of diet-induced hyperhomocysteinemia (HHcy). However, little is known about Hcy-mediated osteoblast dysfunction via mitochondrial oxidative damage. Hydrogen sulfide (H2 S) has potent antioxidant, anti-inflammatory, and antiapoptotic effects. In this
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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