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Key Documents

N9389

Sigma-Aldrich

4-硝基苯基磷酸盐 二钠盐 六水合物

chromogenic, tablet

同義詞:

4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物

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About This Item

線性公式:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS號碼:
分子量::
371.14
Beilstein:
5324661
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

4-硝基苯基磷酸盐 二钠盐 六水合物, tablet

描述

5 mg substrate per tablet

品質等級

形狀

tablet

螢光

λex 310 nm (pH 7.1)
λex 399 nm (Reaction Product)

儲存溫度

−20°C

SMILES 字串

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI 密鑰

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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相關類別

一般說明

用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应,并在 405nm 下读数。

應用

对硝基苯磷酸二钠盐六水合物已用于:
  • 酶联免疫吸附测定(ELISA),用于可视化糖蛋白VI (GPVI)与I型胶原蛋白(Col I)的结合
  • 酶催化显色反应,用于通过ELISA法半定量检测过敏原特异性IgE
  • 碱性磷酸酶检测

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Xiaohong Wang et al.
Biochemical pharmacology, 102, 97-106 (2016-01-06)
Inorganic polyphosphate (polyP) is a physiological polymer composed of tens to hundreds of phosphate units linked together via phosphoanhydride bonds. Here we compared the biological activity of polyP (chain length of 40 phosphate units), complexed with Gd(3+) (polyP·Gd), with the
Marianne K Schesny et al.
Biomaterials, 35(25), 7180-7187 (2014-05-31)
Chemokine-induced stem cell recruitment is a promising strategy for post myocardial infarction treatment. Injection of stromal cell-derived factor 1 (SDF1) has been shown to attract bone marrow-derived progenitor cells (BMPCs) from the blood that have the potential to differentiate into
Mayra Araguaia Pereira Figueiredo et al.
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 27(3), 363-376 (2018-08-02)
The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs
Christina Papista et al.
Kidney international, 88(2), 276-285 (2015-03-26)
IgA1 complexes containing deglycosylated IgA1, IgG autoantibodies, and a soluble form of the IgA receptor (sCD89), are hallmarks of IgA nephropathy (IgAN). Food antigens, notably gluten, are associated with increased mucosal response and IgAN onset, but their implication in the
Masaya Yokota et al.
Arthritis research & therapy, 16(2), R72-R72 (2014-03-19)
In addition to the pivotal roles of mast cells in allergic diseases, recent data suggest that mast cells play crucial roles in a variety of autoimmune responses. However, their roles in the pathogenesis of autoimmune skeletal muscle diseases have not

文章

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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