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N2765

Sigma-Aldrich

4-硝基苯基磷酸盐 二钠盐 六水合物

chromogenic, tablet

同義詞:

4-硝基苯磷酸二钠 六水合物, pNPP 二钠 六水合物, 对硝基苯磷酸二钠, 对硝基苯磷酸盐 二钠 六水合物

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About This Item

線性公式:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS號碼:
333338-18-4
分子量：:
371.14
Beilstein:
5324661
EC號碼:
224-246-5
MDL號碼:
MFCD00066288
分類程式碼代碼:
12352204
PubChem物質ID:
24897592
NACRES:
NA.83

product name

4-硝基苯基磷酸盐 二钠盐 六水合物, tablet

形狀

tablet

品質等級

300

溶解度

water, high purity: 0.25 tablet/mL, clear, colorless

儲存溫度

−20°C

SMILES 字串

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI 密鑰

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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相關類別

一般說明

用于测定酸性和碱性磷酸酶的显色底物。该反应产生黄色的可溶性最终产物。可以用 3N NaOH 终止反应，并在 405nm 下读数。

應用

4-硝基苯磷酸二钠盐六水合物已用于：
  • 在酶联免疫吸附测定(ELISA)中作为碱性磷酸酶偶联抗兔抗体的底物
  • 在酶标免疫实验中作为碱性磷酸酶标记小鼠二抗的底物
  • ELISA法分析动物血清样本中铜绿假单胞菌抗体的存在

數量

每片含有20mg底物

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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