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Merck
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文件

P7998

Sigma-Aldrich

Alkaline Phosphatase Yellow

alkaline phosphatase substrate, chromogenic liquid

同義詞:

Alkaline phosphatase substrate solution (pNPP)

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About This Item

分類程式碼代碼:
12352204
eCl@ss:
42010105
NACRES:
NA.83

product name

用于ELISA的碱性磷酸酶黄色（pNPP）液体底物系统, ready to use solution

品質等級

200

形狀

liquid

儲存溫度

2-8°C

相關類別

一般說明

本品是由 对硝基苯基磷酸酯（pNPP）组成的缓冲碱性磷酸酶底物。这个先进的基质系统含有不溶性稳定剂，可提高稳定性和反应性，并降低基线吸收。它产生一种可溶性终产物，因此不推荐用于蛋白质印迹或组织化学应用。

應用

产生一种可溶性黄色反应产物，可在405nm处读取。对于终点测定，可以使用3N NaOH终止反应。推荐用于ELISA（微孔）分析；不建议用于膜应用。

注意

 可在2-8°C下稳定保存至少一年。

外觀

即用型

準備報告

含有对硝基苯基磷酸酯，它是碱性磷酸酶的底物。

象形圖

Health hazardCorrosion
GHS08,GHS05

訊號詞

Danger

危險聲明

H315,H318,H373

危險分類

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Cristina A Teixeira et al.
Clinical science (London, England : 1979), 130(18), 1665-1675 (2016-07-08)
Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition
Laura C Jacques et al.
Nature communications, 11(1), 1892-1892 (2020-04-22)
Streptococcus pneumoniae serotype 1 is the predominant cause of invasive pneumococcal disease in sub-Saharan Africa, but the mechanism behind its increased invasiveness is not well understood. Here, we use mouse models of lung infection to identify virulence factors associated with
Hannah M Rowe et al.
Infection and immunity, 87(10) (2019-07-17)
Acute otitis media is one of the most common childhood infections worldwide. Currently licensed vaccines against the common otopathogen Streptococcus pneumoniae target the bacterial capsular polysaccharide and confer no protection against nonencapsulated strains or capsular types outside vaccine coverage. Mucosal
Molhm Nassir et al.
Organic & biomolecular chemistry, 17(46), 9913-9923 (2019-11-14)
Nucleotide pyrophosphatase/phosphodiesterase-1 (NPP1) inhibitors have been suggested as a potential treatment for calcium pyrophosphate dihydrate (CPPD) deposition disease. Here, we targeted the development of improved NPP1 inhibitors based on acyclic mimics of Pα,α-phosphorodithioate-substituted adenine nucleotides, 7-10. The latter were obtained
Ortal Danino et al.
Rheumatology (Oxford, England), 57(8), 1472-1480 (2018-04-25)
Calcium pyrophosphate deposition (CPPD) is associated with osteoarthritis and is the cause of a common inflammatory articular disease. Ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (eNPP1) is the major ecto-pyrophosphatase in chondrocytes and cartilage-derived matrix vesicles (MVs). Thus, eNPP1 is a principle contributor to
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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