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N7653

Sigma-Aldrich

对硝基苯磷酸盐液体底物系统

alkaline phosphatase substrate, liquid

同義詞:

4-硝基苯磷酸二钠 溶液, pNPP

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About This Item

線性公式:
O2NC6H4OP(O)(ONa)2
CAS號碼:
4264-83-9
分子量：:
263.05
MDL號碼:
MFCD00007319
分類程式碼代碼:
12352204
PubChem物質ID:
24897835
NACRES:
NA.83

產品名稱

对硝基苯磷酸盐液体底物系统, liquid

形狀

liquid

品質等級

200

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI 密鑰

VIYFPAMJCJLZKD-UHFFFAOYSA-L

相關類別

一般說明

对硝基苯磷酸盐（pNPP）液体底物系统将对硝基苯磷酸盐、缓冲液和所需的镁阳离子结合在一起，成为一种便捷的即用型单一溶液试剂。pNPP能够产生可溶性终产物，是碱性磷酸酶免疫测定的理想底物。

應用

在碱性磷酸酶测定中，采用p-对硝基苯基磷酸盐液体底物体系作为测定碱性磷酸酶活性的底物。
碱性磷酸酶的底物，能够产生可在405 nm处读取的可溶性黄色终产物。使用3N NaOH可终止反应并在405 nm处读取结果。推荐用于ELISA（微孔）分析，不建议用于膜应用。

生化／生理作用

4-硝基苯基磷酸二钠盐六水(pNPP)是测定蛋白质酪氨酸磷酸酶活性的非蛋白质和非特异性底物。pNPP水解后会生成p-硝基酚。它是碱性磷酸酶常见的显色底物。

注意

可在低于0°C的环境中稳定储存至少一年。

外觀

即用型。

象形圖

Health hazardCorrosion
GHS08,GHS05

訊號詞

Danger

危險分類

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

標靶器官

Kidney,Liver,Blood

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
SLCL1721
SLCF2308
SLBP4121V
SLCB1535
SLBV6142

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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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