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Sigma-Aldrich

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

同義詞:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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About This Item

線性公式:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS號碼:
68189-42-4
分子量：:
461.36
Beilstein:
1578142
EC號碼:
269-198-6
MDL號碼:
MFCD00037009
分類程式碼代碼:
12352204
PubChem物質ID:
57652488
NACRES:
NA.32

product name

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐, for the determination of phosphatase, ≥97.0% (enzymatic)

化驗

≥97.0% (enzymatic)

形狀

powder or crystals

品質

for the determination of phosphatase

雜質

≤0.1% free nitrophenol
≤6% water

溶解度

H2O: 0.1 g/mL, clear

儲存溫度

2-8°C

SMILES 字串

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI 密鑰

XXAXKCWOTRABOW-UHFFFAOYSA-N

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相關類別

應用

4-硝基苯磷酸盐 双（三羟甲基氨基甲烷）盐被用于测定细胞裂解后酸性和碱性磷酸酶的酶活性。

生化／生理作用

4-硝基苯磷酸盐是一种非蛋白质和非特异性的显色底物， 是酶联免疫吸附试验（ELISA）中碱性磷酸酶（ALP）的首选。它广泛应用于测定蛋白酪氨酸磷酸酶（PTPs）。酶切后，底物产生黄色产物，可以采用分光光度计测定。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
Aneta Pietraszek et al.
Colloids and surfaces. B, Biointerfaces, 173, 1-8 (2018-09-28)
Alkaline phosphatase (ALP) was sucessfully incorporated into the halloysite (HAL) nanotubes, as confirmed by FTIR-ATR and XRD analyses. The loading efficiency (LE) of ALP was found to be 13.5%, while its encapsulation efficiency (EE) was estimated to be around 27%
O.H. Lowry
Methods in Enzymology, 4, 366-366 (1957)
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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