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MITOISO3

Sigma-Aldrich

酵母线粒体分离试剂盒

sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

用途

sufficient for 40 applications (using 20 OD culture preparations)

儲存溫度

−20°C

一般說明

作为真核细胞能产生能量的地点,线粒体的特征是双层膜结构:外膜和折叠的内膜。分离所得的线粒体可用作进行体外研究的工具,如呼吸和能量生成研究,凋亡、mtDNA和mtRNA、以及线粒体蛋白谱分析。

應用

该试剂盒包含通过溶壁酶裂解酵母细胞壁(原生质球形成),然后进行细胞膜裂解/破碎和线粒体分离所需的所有试剂。另外,该试剂盒包括用于蛋白质组学研究的线粒体蛋白质谱分析提取缓冲液和用于完整线粒体的存储缓冲液。酵母线粒体分离试剂盒已被用于线粒体的分离和提取。

特點和優勢

• 该试剂盒具有快速、灵敏、易用、特异性强的特征
• 该试剂盒含有通过溶细胞酶(lyticase)(微球形式)轻松、快速地溶解细胞壁,以及之后的细胞膜裂解/破坏及线粒体分离所需的一切试剂。
• 该试剂盒为细胞膜破碎提供了两种替代方法。 第一种方法是降解,第二种方法是均质化。
• 该试剂盒包含一份可用于线粒体蛋白谱分析的提取缓冲液,可供蛋白质组研究使用
• 该试剂盒包含用于完整线粒体的储液。
• 所含的试剂足够进行40次20 OD培养物的处理
• 该试剂盒已在Saccharomyces cerevisiaePichia pastorisSchizosaccharomyces pombe上进行过测试

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • L2524Lyticase from Arthrobacter luteus, lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuretSDS

  • P8215Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solutionSDS

  • C0356Protein Extraction Reagent Type 4SDS

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2

儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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