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Merck
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重要文件

P1644

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (20 °C)

同義詞:

Percoll密度梯度介质

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

aseptically filled

品質等級

形狀

colloidal ((Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)) )

技術

cell culture | mammalian: suitable

顏色

clear colorless to faint yellow

pH值

8.5-9.5 (20 °C)

應用

hematology
histology

儲存溫度

2-8°C

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一般說明

Percoll®是一种用于细胞、病毒和亚细胞颗粒密度梯度离心的经典培养基。Percoll由直径为15-30 nm(在水中为23% w/w)并涂覆有聚乙烯吡咯烷酮(PVP)的胶体二氧化硅颗粒组成。PVP涂层使产品完全无毒,非常适合与生物材料一起使用。

應用

Percoll®已用于在精子准备中以研究牛胚胎的能量需求。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用于细胞分离和细胞器分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度,低渗透压密度梯度,可用于分离细胞,细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的,无毒,并且很容易从纯化的材料中去除。

法律資訊

Percoll is a registered trademark of Cytiva

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Rajasree Sreedharan et al.
Kidney international, 86(3), 515-524 (2014-05-09)
Inducible heat shock proteins (HSPs), regulated by heat shock factor-1 (HSF-1), protect against renal cell injury in vitro. To determine whether HSPs ameliorate ischemic renal injury in vivo, HSF-1 functional knockout mice (HSF-KO) were compared with wild-type mice following bilateral
T Hirano et al.
The Journal of cell biology, 106(4), 1171-1183 (1988-04-01)
A temperature-sensitive mutant nuc2-663 of the fission yeast Schizosaccharomyces pombe specifically blocks mitotic spindle elongation at restrictive temperature so that nuclei in arrested cells contain a short uniform spindle (approximately 3-micron long), which runs through a metaphase plate-like structure consisting
Guilherme D Melo et al.
Scientific reports, 7(1), 8454-8454 (2017-08-18)
Visceral leishmaniasis (VL) is a systemic disease with multifaceted clinical manifestations, including neurological signs, however, the involvement of the nervous system during VL is underestimated. Accordingly, we investigated both brain infection and inflammation in a mouse model of VL. Using
Tanja Zöller et al.
International journal of molecular sciences, 19(3) (2018-03-02)
Microglia are the resident immune cells of the central nervous system (CNS) and participate in physiological and pathological processes. Their unique developmental nature suggests age-dependent structural and functional impairments that might contribute to neurodegenerative diseases. In the present study, we
R Hovius et al.
Biochimica et biophysica acta, 1021(2), 217-226 (1990-01-29)
Rat liver mitochondria were isolated by a combination of differential and Percoll gradient centrifugation, resulting in a highly pure and intact preparation, as assessed by marker enzyme analysis, latency of cytochrome-c oxidase, respiratory control index and electron microscopy. Two different

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