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Key Documents

P7828

Sigma-Aldrich

Percoll®

suitable for plant cell culture

同義詞:

density gradient medium

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About This Item

MDL號碼:
分類程式碼代碼:
10171502
NACRES:
NA.72

無菌

aseptically filled

品質等級

技術

cell culture | plant: suitable

pH值

8.9 (20 °C)

應用

agriculture

儲存溫度

2-8°C

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應用

Percoll ® 已用于密度梯度离心分离绿藻 、蜕膜基质细胞 和公鸡精子的不同种群的完整叶绿体。
Percoll 用于植物研究中的原生质体和细胞器(叶绿体、线粒体)分离。
Percoll由粒径为15-30nm(在水中为23% w/w)的硅胶颗粒组成,这些颗粒被聚乙烯吡咯烷酮(PVP)包覆。 它被用来建立无毒低粘度,低渗透压密度梯度,可用于分离细胞,细胞器和病毒。它已被用来分离 大肠杆菌的内外膜泡。 Percoll在整个梯度过程中都是等渗的,无毒,并且很容易从纯化的材料中去除。

法律資訊

Percoll is a registered trademark of Cytiva

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


分析證明 (COA)

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存取文件庫

Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Ahammad MU, et al.
Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy
He YY, et al.
Clinical Immunology (Orlando, Fla.), 145(2), 161-173 (2012)
Manuel Guinea Díaz et al.
Nature communications, 9(1), 50-50 (2018-01-05)
Activation of the plastid-encoded RNA polymerase is tightly controlled and involves a network of phosphorylation and, as yet unidentified, thiol-mediated events. Here, we characterize PLASTID REDOX INSENSITIVE2, a redox-regulated protein required for full PEP-driven transcription. PRIN2 dimers can be reduced into

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