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Merck
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重要文件

F2637

Sigma-Aldrich

Ficoll® 400

BioXtra, for molecular biology, lyophilized powder

同義詞:

多聚蔗糖 400

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.52

生物源

non-animal source

品質等級

等級

Molecular Biology
for molecular biology

產品線

BioXtra

形狀

lyophilized powder

異物活動

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

儲存溫度

room temp

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一般說明

Ficoll® 400是蔗糖的非离子合成聚合物,可用于细胞分离和器官分离。

應用

用于细胞分离和细胞器分离。
适用于
  • 杂交实验,以减少标记探针与膜的非特异性结合
  • 制备DNA上样缓冲液(15% Ficoll®,0.25%溴酚蓝和0.25%二甲苯蓝FF)
  • 分离密度相似但大小不同的细胞(来自人外周血、骨髓和脐带血的PBMC)
  • 产生密度梯度,以便分离亚细胞成分
  • 细胞和组织样品的玻璃化。
  • 比较不同灌注液对缺氧性肺血管收缩(HPV)的影响。

特點和優勢

  • 不含DNA酶、RNA酶、磷酸酶和蛋白酶
  • 可保留分离细胞的功能和形态完整性

其他說明

一种非离子型合成蔗糖聚合物。

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Gaye Cetinkaya et al.
Cryobiology, 63(3), 292-297 (2011-10-25)
Preservation of cell and tissue samples from endangered species is a part of biodiversity conservation strategy. Therefore, setting up proper cell and tissue cryopreservation methods is very important as these tissue samples and cells could be used to reintroduce the
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Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
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