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Merck
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F2878

Sigma-Aldrich

Ficoll® PM 70

Type 70

同義詞:

聚(蔗糖--表氯醇)

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

種類

Type 70

品質等級

形狀

powder

分子量

~70,000

顏色

white

溶解度

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

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一般說明

Ficoll® PM70是一种高分子量支化多糖聚合物,公认不含任何离子化基团。这种蔗糖聚合物由蔗糖与环氧氯丙烷共聚形成。

應用

Ficoll® PM 70已用于:
  • 用作比较研究中的大分子,评估其在玻璃化溶液中的效果
  • 用作卵母细胞玻璃化过程的玻璃化溶液补充剂
  • 用作人肺成纤维细胞培养的低血清培养基成分,促进形成拥挤环境

生化/生理作用

Ficoll 常用作玻璃化溶液的大分子成分。由于其密集的分支,Ficolls也可用作理想的近球形分子拥挤剂。
在细胞体积信号转导和蛋白质重折叠研究中,FicollTM PM 70用作大分子拥挤剂。这一物质可用于组织工程和大分子构象研究,开发、评估和使用大分子拥挤 (MMC) 系统和配置。 Ficoll PM-70还可用于玻璃化研究。

其他說明

蔗糖的非离子合成聚合物。
为了全面了解我们针对客户研究提供的各种多糖产品,建议您访问我们的碳水化合物分类页面。

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Emmanuel Dauty et al.
Journal of molecular recognition : JMR, 17(5), 441-447 (2004-09-14)
Aqueous environments in living cells are crowded, with up to >50 wt% small and macromolecule-size solutes. We investigated quantitatively one important consequence of molecular crowding--reduced diffusion of biologically important solutes. Fluorescence correlation spectroscopy (FCS) was used to measure the diffusion
Nobuhiko Tokuriki et al.
Protein science : a publication of the Protein Society, 13(1), 125-133 (2003-12-24)
Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in
M B Burg
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 10(5-6), 251-256 (2000-12-23)
The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are
M Al-Habori
The international journal of biochemistry & cell biology, 33(9), 844-864 (2001-07-20)
Macromolecular crowding has been proposed as a mechanism by means of which a cell can sense relatively small changes in volume or, more accurately, the concentration of intracellular solutes. According to the macromolecular theory, the kinetics and equilibria of enzymes
C M Checura et al.
Theriogenology, 67(5), 919-930 (2006-12-19)
This study was designed to evaluate vitrification procedures for in vitro matured bovine oocytes for efficient blastocyst production after warming, IVF and culture. A second goal was to replace serum as the macromolecular component of the vitrification solution, without compromising

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