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Key Documents

GE17-0300-10

Ficoll® PM 400

Cytiva 17-0300-10, pack of 100 g

同義詞:

Density gradient medium

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About This Item

分類程式碼代碼:
12352201
NACRES:
NA.25

形狀

solid
spray-dried

包裝

pack of 100 g

製造商/商標名

Cytiva 17-0300-10

顏色

White

溶解度

soluble

一般說明

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.
It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

特點和優勢

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
  • Better osmotic properties than sucrose.
  • Preserves functional and morphological integrity.
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
  • Does not penetrate biological membranes.
  • Serves as the raw material for preparation of Ficoll-Paque gradients.
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

13 - Non Combustible Solids


分析證明 (COA)

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文章

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

This page shows methods to remove Percoll from a sample after centrifugation.

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

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How to use percoll to prepare a gradient

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