推薦產品
生物源
bovine pancreas
品質等級
形狀
lyophilized
包裝
pkg of 100 mg
製造商/商標名
Roche
技術
DNA extraction: suitable
最適pH
~7.0
儲存溫度
2-8°C
一般說明
牛胰腺脱氧核糖核酸酶I(DNase I)是DNA小沟相互作用的核酸酶,特异性相对较低。该蛋白质由两个中心β 折叠构成,而每个折叠由六个β-链所组成。该结构被大量的环以及α-螺旋区域包围。该酶与核酸外切酶III具有结构相似性。DNase I是研究得最透彻的哺乳动物来源的内切酶之一。它是一种双链特异性内切核酸酶,需要二价阳离子激发最大活性。
應用
蛋白质(如膜蛋白)的分离操作。
牛胰腺脱氧核糖核酸酶I(DNase I)已被用于:
牛胰腺脱氧核糖核酸酶I(DNase I)已被用于:
- 分离肺、皮肤和肿瘤样品中的细胞
- 从抗DNase I的活细菌细胞中提取细菌DNA †
單位定義
一个单位表示在测定条件下导致吸光度以0.001每分钟的速率而增加的酶活力。
準備報告
激活剂:Ca2+ (0.12 mM)与Mg2+结合在一起可增强活性 [Laskowski (1971); Melgar及Goldthwait (1968)].
工作浓度:1 mg/ml
在溶解过程中请勿涡旋!
储备条件 (工作溶液):当在水中重悬时,溶液可在2至8 °C下保存2至3天。在溶解过程中请勿涡旋。注:至少以1 mg/ml溶解。
工作浓度:1 mg/ml
在溶解过程中请勿涡旋!
储备条件 (工作溶液):当在水中重悬时,溶液可在2至8 °C下保存2至3天。在溶解过程中请勿涡旋。注:至少以1 mg/ml溶解。
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
does not flash
閃點(°C)
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Nature, 583(7817), 609-614 (2020-06-26)
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer, but they have only modest efficacy and limited tolerability1,2. In an effort to identify alternative cytokine pathways for immunotherapy, we found that components of the
Applied and environmental microbiology, 79(19), 5936-5941 (2013-07-23)
Human lungs are constantly exposed to bacteria in the environment, yet the prevailing dogma is that healthy lungs are sterile. DNA sequencing-based studies of pulmonary bacterial diversity challenge this notion. However, DNA-based microbial analysis currently fails to distinguish between DNA
Bio-protocol, 10(6), e3551-e3551 (2021-03-05)
Nucleotide-sugar transporters (NSTs) facilitate eukaryotic cellular glycosylation by transporting nucleotide-sugar conjugates into the Golgi lumen and endoplasmic reticulum for use by glycosyltransferases, while also transferring nucleotide monophosphate byproducts to the cytoplasm. Mutations in this family of proteins can cause a
American journal of cancer research, 4(1), 29-41 (2014-02-01)
Insulin-like growth factor binding protein 3 (IGFBP3), a hypoxia-inducible gene, regulates a variety of cellular processes including cell proliferation, senescence, apoptosis and epithelial-mesenchymal transition (EMT). IGFBP3 has been linked to the pathogenesis of cancers. Most previous studies focus upon proapoptotic
Clinical and experimental dermatology, 41(5), 552-556 (2016-01-26)
Studying skin immune cells under various pathophysiological conditions is vital for understanding the nature of cutaneous inflammatory responses. Available methods of isolating cells from the skin have relatively low yield or require in vitro culture. To increase the effective isolation
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務