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CS0390

Sigma-Aldrich

线粒体染色试剂盒

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

同義詞:

JC-1 dye

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32

品質等級

200

用途

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

包裝

pkg of 1 kit

儲存條件

dry at room temperature

技術

flow cytometry: suitable
protein staining: suitable

螢光

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

應用

cell analysis
detection

檢測方法

fluorometric

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

一般說明

在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何消耗线粒体跨膜电位的活动（如凋亡）都会影响线粒体中的JC-1染料聚集，导致这种染料分散到整个细胞中，从而使红色荧光（JC-1聚集体）转变为绿色荧光（JC-1单体）。本试剂盒可进行细胞染色，再通过荧光显微镜观察或流式细胞仪观测或测定荧光强度。

應用

线粒体电化学势梯度 (Δψ) 消耗被视为细胞凋亡的早期症状。本试剂盒采用阳离子亲脂性染料JC-1，可在活细胞中方便、快速地检测线粒体内膜电化学电位变化。

特點和優勢

试剂盒提供：
  • 可简单、快速、方便地染色和分析线粒体完整性
  • 包含检测线粒体内膜电化学势变化所需的全部试剂
  • 包含抗生素缬氨霉素，可作为防止JC-1聚集的对照试剂
  • 可在绿色和红色通道中清晰地检测到荧光变化
  • 适用于贴壁细胞和悬浮细胞
  • 已经过Jurkat、U-937、HeLa和NIH3T3细胞的测试
  • 可通过荧光显微镜或流式细胞仪观测和分析荧光信号

僅套裝組件

產品號碼
描述
  • DMSO 1 mL
  • JC Staining Buffer 5X 120 mL
  • JC-1 1 mg
  • Valinomycin Ready Made .1 mL

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Small Molecule Mediated Restoration of Mitochondrial Function Augments Anti-Mycobacterial Activity of Human Macrophages Subjected to Cholesterol Induced Asymptomatic Dyslipidemia.
Suman Asalla et al.
Frontiers in cellular and infection microbiology, 7, 439-439 (2017-10-27)
Robert L Elliott et al.
PloS one, 14(4), e0214586-e0214586 (2019-04-02)
Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life
Maria C Zanellati et al.
Frontiers in genetics, 6, 78-78 (2015-03-31)
Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed
Whasun Lim et al.
Journal of cellular physiology, 231(12), 2690-2699 (2016-03-13)
Apigenin is a flavonoid found in parsley, onions, oranges, tea, chamomile, wheat, and sprouts. It has a variety of biological properties including anti-oxidant, anti-mutagenic, anti-carcinogenic, anti-inflammatory, anti-proliferative, and anti-spasmodic effects. Based on epidemiological and case-control studies, apigenin is regarded as
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
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