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MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

同義詞:

Mitochondria Purification Kit

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About This Item

EC號碼:
232-650-8
分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

200

用途

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

儲存期限

2 yr

技術

fractionation: suitable

運輸包裝

dry ice

儲存溫度

−20°C

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相關類別

應用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分，并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于：
  • 分离线粒体和胞浆组分，用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体，检测线粒体跨膜电位

特點和優勢

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂，即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDS

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象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

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Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Enrique Santamaria et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(6), 3065-3070 (2003-03-13)
Recent work shows that S-adenosylmethionine (AdoMet) helps maintain normal liver function as chronic hepatic deficiency results in spontaneous development of steatohepatitis and hepatocellular carcinoma. The mechanisms by which these nontraditional functions of AdoMet occur are unknown. Here, we use knockout
Mithu Guha et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(8), 1224-1226 (2006-04-11)
Hepatic dysfunction is a common clinical complication in malaria, although its pathogenesis remains largely unknown. Using a variety of in vivo and ex vivo approaches, we have shown for the first time that malarial infection induces hepatic apoptosis through augmentation
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文章

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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