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CYTOCOX1

Sigma-Aldrich

细胞色素c氧化酶检测试剂盒

sufficient for 100 tests, soluble and membrane bound mitochondria

同義詞:

Cytochrome c Oxidase Activity Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

200

用途

sufficient for 100 tests

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... COX1(4512)

相關類別

應用

细胞色素c氧化酶检测试剂盒使用了一种经过优化的比色检测方法，该方法基于550 nm处测得的高铁细胞色素c吸光度下降（这是由于高铁细胞色素c被细胞色素c氧化酶氧化造成的）进行检测。 该试剂盒适于检测线粒体外膜完整度/线粒体应激性，同时也适于检测亚细胞组分之中的线粒体。

生化／生理作用

细胞色素c氧化酶[EC 1.9.3.1]位于线粒体内膜上,将线粒体基质和膜间隙分离开来，一直被用作这种膜的标记物。在细菌的细胞质膜上，也存在细胞色素c氧化酶。细胞色素c氧化酶可通过氧化磷酸化过程连接细胞色素链之间的电子转运，为细胞提供能量。

特點和優勢

  • 简单，经优化的实验方案 - 无需进行专门的培训，即可获得可重复的结果
  • 可用于测定任意线粒体来源的细胞色素酶c的活性 - 这种酶在所有种类生物的线粒体中均存在
  • 可用于检测亚细胞成分中是否存在线粒体 - 节省时间，同时提高了细胞器制备质量的可信度
  • 可结合MITOISO1 Mitochondrial Isolation Kit使用- 标准化的线粒体制备和分析，确保了可重复性
  • 可用于分析线粒体膜的完整性
  • 简单的比色测定解决方案

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H315,H318

危險分類

Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Céline Aguer et al.
PloS one, 6(12), e28536-e28536 (2011-12-24)
Human primary myotubes are highly glycolytic when cultured in high glucose medium rendering it difficult to study mitochondrial dysfunction. Galactose is known to enhance mitochondrial metabolism and could be an excellent model to study mitochondrial dysfunction in human primary myotubes.
Filip J Larsen et al.
Cell metabolism, 13(2), 149-159 (2011-02-03)
Nitrate, an inorganic anion abundant in vegetables, is converted in vivo to bioactive nitrogen oxides including NO. We recently demonstrated that dietary nitrate reduces oxygen cost during physical exercise, but the mechanism remains unknown. In a double-blind crossover trial we
Hikaru Miyagi et al.
The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p, rather than the NAD kinase reaction followed by the NADP(+)-dependent dehydrogenase reaction, had been
Uta Keil et al.
The Journal of biological chemistry, 279(48), 50310-50320 (2004-09-17)
Increasing evidence suggests an important role of mitochondrial dysfunction in the pathogenesis of Alzheimer's disease. Thus, we investigated the effects of acute and chronic exposure to increasing concentrations of amyloid beta (Abeta) on mitochondrial function and nitric oxide (NO) production
Chunpeng Xu et al.
Frontiers in behavioral neuroscience, 12, 238-238 (2018-10-26)
The habenula is a nuclear complex composed of the lateral habenula (LHb) and medial habenula (MHb), two distinct structures. Much progress has been made to emphasize the role of the LHb in the pathogenesis of depression. In contrast, relatively less
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