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Key Documents

GL0010

Sigma-Aldrich

高尔基体分离试剂盒

sufficient for 50 g (tissue)

同義詞:

Golgi Kit, Isolation Kit for Golgi

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

用途

sufficient for 50 g (tissue)

品質等級

技術

fractionation: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

高尔基体分离试剂盒提供了一种通过不连续密度梯度分离法从哺乳动物软组织中分离高尔基膜的方法。通过测定UDP-半乳糖转移酶的活性,或者采用适当的抗体免疫检测B-COP或GM130等高尔基体特异性标志物蛋白,来测定高尔基体的富集程度(货号:分别为G6160和G7295)。其他细胞器的分离可采用Sigma提供的相应标志物检测试剂盒检测(货号:CS0780、CYTOCOX1、CY0100和CAT100)。

應用

高尔基体分离试剂盒可用于通过不连续密度梯度分离法从哺乳动物软组织中分离高尔基膜。

分析報告

这一款高尔基体分离试剂盒采用大鼠肝脏样本进行了优化,并在大鼠肾脏、脾脏和心脏上进行过测试。

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLSDS

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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E Prchla et al.
The Journal of cell biology, 131(1), 111-123 (1995-10-01)
Endosomal penetration by nonenveloped viruses might be accomplished by either local breakdown of the endosomal membrane (e.g., adenovirus) or formation of a membrane-spanning pore by capsid proteins. Uncoating of the nonenveloped virus human rhinovirus serotype 2 (HRV2) has been shown
Martin J Lear et al.
Journal of natural products, 72(11), 1980-1987 (2009-10-22)
(+/-)-Laetirobin (1) was isolated as a cytostatic lead from Laetiporus sulphureus growing parasitically on the black locust tree, Robinia pseudoacacia, by virtue of a reverse-immunoaffinity system. Using an LC/MS procedure, milligram quantities of (+/-)-laetirobin (1) were obtained, and the structure
E R Sjoberg et al.
The Journal of biological chemistry, 268(14), 10185-10196 (1993-05-15)
The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized. However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure. Here, we have studied the spatial and temporal interrelationships among the
Xiaoqing Xu et al.
Cell, 175(5), 1336-1351 (2018-10-16)
As a critical step during innate response, the cytoplasmic β subunit (IFN-γR2) of interferon-γ receptor (IFN-γR) is induced and translocates to plasma membrane to join α subunit to form functional IFN-γR to mediate IFN-γ signaling. However, the mechanism driving membrane
Julien Villeneuve et al.
The Journal of cell biology, 217(2), 649-665 (2017-12-08)
An appreciation of the functional properties of the cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with the recent demonstration that an extracellular form secreted by adipocytes regulates a wide range of physiological functions. Little, however, is known about

文章

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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