推薦產品
用途
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
儲存期限
1 yr at 2‑8 °C
包裝
pkg of 1 kit
應用
cell analysis
異物活動
nuclease and protease, free
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。
應用
适于作为细胞核成分的来源，为体外凋亡检测和功能研究提供细胞核成分。
生化／生理作用
配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核，包括贴壁 （例如，HEK293和COS7）和非贴壁（例如，Jurkat和HFN7.1）细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
其他說明
所有操作步骤应在冰块上或2-8°C条件下进行。
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
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