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Key Documents

MAK159

Sigma-Aldrich

线粒体膜电位检测试剂盒(适用于酶标仪)

sufficient for 500 fluorometric tests (microplate readers)

同義詞:

JC-10检测, JC-10线粒体膜蛋白检测

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.25

用途

sufficient for 500 fluorometric tests (microplate readers)

檢測方法

fluorometric

相關疾病

cancer

儲存溫度

−20°C

一般說明

由于电子传递链上酶的活动,线粒体在其膜上产生电位。在细胞凋亡过程中,线粒体膜电位(MMP)的崩溃与线粒体通透性转换孔的开放一致,导致细胞色素c 释放到细胞质中,进而引发细胞凋亡级联反应的其他下游事件。

應用

线粒体膜电位试剂盒已用于测量线粒体膜电位。

適合性

本试剂盒适于检测哺乳动物细胞中的线粒体跨膜电势差(MMP),并可用于在酶标仪上筛选凋亡抑制剂及激活剂等。

原則

该试剂盒利用 JC-10,一种优于JC-1的替代品,用于测定细胞中MMP的损失。尽管 JC-1在许多实验室中被广泛使用,但是当其在较高浓度下使用时,其水溶性差通常会导致水性缓冲液沉淀。在较高浓度下,JC-10 表现出比 JC-1 更高的水溶性。与JC-1 类似,JC-10是一种阳离子亲脂性染料,其浓缩并在具有极化线粒体膜的细胞线粒体中形成可逆的红色荧光 JC-10 聚集体(λex = 540/λem = 590 nm)。在凋亡细胞中,MMP 崩溃导致 JC-10 无法保留在线粒体中,并使染料返回到单体绿色荧光形式(λex = 490/λem = 525 nm)。该试剂盒可用于监测细胞凋亡和筛选凋亡抑制剂和激活剂。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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