推薦產品
一般說明
组蛋白脱乙酰化酶（HDAC）是从组蛋白中去除乙酰基的一大类酶。 已显示位点特异性组蛋白乙酰化和脱乙酰化分别激活或抑制真核基因转录，因此，它在哺乳动物发育和疾病中起关键作用。HDACs参与重要的生物学活动，例如细胞分化，增殖，凋亡和衰老。
使用Sigma′s HDAC3活性测定试剂盒，试样中存在的HDAC3将与提供的显影剂一起作用，使其脱乙酰化，然后裂解HDAC3底物[R-H-K-K（Ac）-AFC]。 该活性将释放淬灭的荧光基团AFC，可以在Em/Ex=380/500 nm处检测到。 曲古抑菌素A是试剂盒中包含的HDAC抑制剂，用于验证HDAC3活性。该试剂盒提供了快速，简单，灵敏和可靠的测试。 它适用于来自核提取物，纯化或免疫沉淀的HDAC3以及天然，重组或基因修饰的HDAC3的单独测试或高通量测定。
使用Sigma′s HDAC3活性测定试剂盒，试样中存在的HDAC3将与提供的显影剂一起作用，使其脱乙酰化，然后裂解HDAC3底物[R-H-K-K（Ac）-AFC]。 该活性将释放淬灭的荧光基团AFC，可以在Em/Ex=380/500 nm处检测到。 曲古抑菌素A是试剂盒中包含的HDAC抑制剂，用于验证HDAC3活性。该试剂盒提供了快速，简单，灵敏和可靠的测试。 它适用于来自核提取物，纯化或免疫沉淀的HDAC3以及天然，重组或基因修饰的HDAC3的单独测试或高通量测定。
特點和優勢
- 简单灵敏可靠的测定
- 程序简单；需要约60分钟
- 使用荧光测定法
- 样品类型：细胞和组织裂解物、血浆和血清、其他生物体液
- 种属反应性：哺乳动物
- 适用于独立测试或高通量分析和动力学研究
- 方便的96孔微孔板规格
- 适用于纯化，免疫沉淀和重组或基因修饰的HDAC3样品中HDAC3活性的高通量测量
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
Karolina J Janczura et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(47), E11148-E11157 (2018-11-07)
Alzheimer's disease (AD) is the leading cause of age-related dementia. Neuropathological hallmarks of AD include brain deposition of β-amyloid (Aβ) plaques and accumulation of both hyperphosphorylated and acetylated tau. RGFP-966, a brain-penetrant and selective HDAC3 inhibitor, or HDAC3 silencing, increases
Ji Heon Noh et al.
Cancer research, 74(6), 1728-1738 (2014-01-23)
Aberrant regulation of histone deacetylase 2 (HDAC2) contributes to malignant progression in various cancers, but the underlying mechanism leading to the activation of oncogenic HDAC2 remains unknown. In this study, we show that HDAC2 expression is upregulated in a large
Astrid M Kral et al.
Biochemistry, 53(4), 725-734 (2014-01-24)
Histone deacetylases (HDACs) play diverse roles in many diseases including cancer, sarcopenia, and Alzheimer's. Different isoforms of HDACs appear to play disparate roles in the cell and are associated with specific diseases; as such, a substantial effort has been made
Johannes Gräff et al.
Cell, 156(1-2), 261-276 (2014-01-21)
Traumatic events generate some of the most enduring forms of memories. Despite the elevated lifetime prevalence of anxiety disorders, effective strategies to attenuate long-term traumatic memories are scarce. The most efficacious treatments to diminish recent (i.e., day-old) traumata capitalize on memory updating
Ouafa Zerzaihi et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 92(1), 61-67 (2014-01-30)
Besides its direct metabolic effects, insulin induces transcriptional alterations in its target tissues. However, whether such changes are accompanied by epigenetic changes on the chromatin template encompassing insulin responsive genes is unclear. Here, mRNA levels of insulin-responsive genes hexokinase 2
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務