推薦產品
生物源
bovine erythrocytes
形狀
lyophilized powder
比活性
≥2,000 W-A units/mg protein
分子量
30 kDa
應用
diagnostic assay manufacturing
儲存溫度
2-8°C
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應用
牛红血球(BCA)来源碳酸酐酶已用于研究去除酶结合金属离子Zn2+对酶的聚集行为的影响。使用螯合剂（例如EDTA（乙二胺四乙酸） ）去除金属离子，增强了酶采用熔融球状态的倾向。人们发现，这种状态的酶可与分子伴侣样α-晶状体蛋白结合并防止聚集。来自Sigma的酶已固定在电化学传感器上，以便开发出彻底溶解CO2的分析装置。其还用于BCA构象变化的热力学分析。
生化／生理作用
碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶催化二氧化碳水合成碳酸。它参与重要的生理和病理过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致肿瘤性。因此，该酶是用于各种病理学（如青光眼、癫痫和帕金森病）的临床应用的抑制剂的重要靶标。
單位定義
在0°C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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