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C0954

Sigma-Aldrich

Chondroitinase C from Flavobacterium heparinum

lyophilized powder, ≥200 units/mg solid

同義詞:

Chondroitin C lyase

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

共軛

(Glucosaminoglycan)

品質等級

形狀

lyophilized powder

比活性

≥200 units/mg solid

儲存溫度

−20°C

一般說明

Enzyme Commission (EC) Number 4.2.2.x

生化/生理作用

Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.

單位定義

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities

文章

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

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