推薦產品
共軛
(Glucosaminoglycan)
品質等級
形狀
lyophilized powder
比活性
0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)
成份
Protein, ~15% Lowry
溶解度
0.02 M phosphate buffer: soluble (pH 7.0)
異物活動
Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount
儲存溫度
−20°C
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應用
Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.
生化／生理作用
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
其他說明
Contains potassium phosphate buffer salts and BSA as stabilizer.
查看有关 用于复合碳水化合物分析的酶 的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
適合性
Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides
單位定義
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).
準備報告
Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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