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H1136

Sigma-Aldrich

透明质酸酶 来源于 Streptomyces hyalurolyticus

同義詞:

透明质酸裂解酶 来源于 Streptomyces hyalurolyticus

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About This Item

CAS號碼:
37259-53-3
酶委員會編號:
4.2.2.1 (BRENDA, IUBMB)
EC號碼:
253-430-8
MDL號碼:
MFCD00131347
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Streptomyces sp. (S. hyalurolyticus)

品質等級

200

共軛

conjugate (Glucosaminoglycan)

形狀

film (Clear)

包裝

ampule of 300 ×  units

儲存溫度

−20°C

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相關類別

一般說明

透明质酸酶广泛存在于自然界。在人体中，它存在于不少器官和体液中。透明质酸酶见于病毒、噬菌体、真菌和细菌的分泌物。它还存在于线虫和蚂蟥的分泌物中。蛇和蝎子的毒液中会产生透明质酸酶。蜜蜂、蜘蛛、黄蜂、大黄蜂、毛虫、鱼类和蜥蜴也会分泌透明质酸酶。

應用

透明质酸酶（来自Streptomyces hyalurolyticus）已用于：
  • 制备DMEM/F-12（杜氏改良Eagle培养基/营养混合物F-12）培养基，用于从分离肿瘤组织中分离和纯化单细胞
  • 作为消化液组分，用于从睾丸组织获得生殖干细胞

生化／生理作用

透明质酸裂解酶在β-D-GlcNAc-(1→4)-β-D-GlcA键上切割透明质酸，得到3-(4-脱氧β-D-葡萄糖-4-烯基糖基)-N-乙酰基-D-葡糖胺四糖和六糖。与其他透明质酸酶不同，该酶对透明质酸具有特异性，并且对软骨素和硫酸软骨素无活性。
透明质酸酶属于内切糖苷酶，通过配合β-葡糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶等外切糖苷酶完成其内切功能。透明质酸酶调节透明质酸的周转代谢。

單位定義

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

其他說明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

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