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Key Documents

A1902

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

同義詞:

Rabbit Anti-Mouse IgG (whole molecule)−AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

rabbit

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

mouse

技術

direct ELISA: 1:1,000
western blot: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.

特異性

Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.

生化/生理作用

IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

外觀

Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

IgG Subclasses and Allotypes: From Structure to Effector Functions
Vidarsson G, et al.
Frontiers in Immunology, 5 (2014)
A M Robinson et al.
Biochimica et biophysica acta, 1369(2), 278-286 (1998-04-29)
Immunoliposomes have been prepared using antibodies raised to an antigenic determinant on the cell surface of the oral bacterium Streptococcus oralis (S. oralis) in an investigation of their potential to reduce dental plaque. The N-succinimidyl-S-acetylthioacetate (SATA) derivative of the antibodies
IgG Placental Transfer in Healthy and Pathological Pregnancies
Palmeira P, et al.
Journal of immunology research, 2012 (2011)
Jianing Li et al.
The Journal of allergy and clinical immunology, 131(2), 442-450 (2012-11-28)
The prevalence of peanut allergies is increasing. Peanuts and many other allergen sources contain significant amounts of triglycerides, which affect absorption of antigens but have unknown effects on sensitization and anaphylaxis. We recently reported that dietary medium-chain triglycerides (MCTs), which
A role for IgG immune complexes during infection with the intracellular pathogen Leishmania
Miles SA, et al.
The Journal of Experimental Medicine, 201(5), 747-747 (2005)

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