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A3937

Sigma-Aldrich

抗-兔IgG(全分子),F(ab′)2 片段−碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

rabbit

技術

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

IgG是糖蛋白抗体,可调控几种免疫应答。靶蛋白的兔IgG通常在多种研究应用中用作一抗。因此,与可检测底物缀合的抗兔IgG二抗是靶蛋白分析的有用工具。抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体结合全部兔免疫球蛋白。

免疫原

纯化兔 IgG

應用

以碱性磷酸酶标记的山羊抗兔F2片段特异性抗体为二抗,在室温下对前列腺上皮细胞裂解液(1:7500稀释)进行2小时蛋白质印迹分析。
成功使用该抗体的应用以及相关同行评审论文如下所示。
酶联免疫吸附试验免疫印迹法(1 篇)
蛋白印迹法(1 篇)
抗兔IgG(全分子),(F(ab′)2)片段-碱性磷酸酶抗体适用于免疫印迹(5μL)和直接ELISA(1:30,000)。该产品也可用于免疫组织化学(1:50,使用福尔马林进行固定、石蜡包埋切片)。

外觀

溶剂为0.05 M Tris缓冲液,pH 8.0,含1 mM MgCl2、10 mM甘氨酸、1%牛血清白蛋白、50%甘油和15 mM叠氮化钠

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Previously, a bovine intestinal epithelial cell line (BIE cells) was successfully established. This work hypothesized that BIE cells are useful in vitro model system for the study of interactions of microbial- or pathogen-associated molecular patterns (MAMPs or PAMPs) with bovine
Ratthaphol Charlermroj et al.
PloS one, 8(4), e62344-e62344 (2013-05-03)
Plant pathogens are a serious problem for seed export, plant disease control and plant quarantine. Rapid and accurate screening tests are urgently required to protect and prevent plant diseases spreading worldwide. A novel multiplex detection method was developed based on

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