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Key Documents

A1418

Sigma-Aldrich

抗 小鼠 IgG(Fc 特异性)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous solution

同義詞:

Goat Anti-Mouse IgG (Fc specific)–AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

重組細胞

expressed in goat

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse

應無反應活性

human

技術

direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

尋找類似的產品? 前往 產品比較指南

一般說明

IgG 抗体在补体激活、吞噬作用、胎盘转运和细胞表面受体结合等体液免疫应答中起关键作用。抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体(以1:10,000 的稀释度采用PBS-Tween 稀释)可用于 ELISA。它也可用于免疫印迹。山羊抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体与小鼠 IgG Fc 片段、IgG 及其亚类 IgG1、IgG2a、IgG2b 和 IgG3 发生特异性反应,但不与人 IgG 和小鼠 IgA 或 IgM 发生反应。

免疫原

纯化的小鼠 IgG Fc 片段。

應用

抗小鼠 IgG(Fc 特异性)-碱性磷酸酶抗体可用于 ELISPOT(免疫斑点)测定。它也可用于免疫组化和蛋白质印迹。
碱性磷酸酶缀合的山羊抗小鼠 Fc 特异性抗体在 ELISA 测定中用作二抗,在 PBS/0.1% Tween 和 1% BSA中以 1:1000 的稀释度,在 37°C 下持续 1.5 小时。该抗体经开发使用磷酸 4-硝基苯酯(Sigma)作为底物,在 37°C 下,持续 30分钟。

其他說明

用人IgG吸附抗体。

外觀

0.05 MTris (pH 8.0) 溶液,含 1% 牛血清白蛋白、1 mM MgCl 2 和 15 mM 叠氮化钠

準備報告

经过吸附以减少人体样本的背景染色。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

D J Lewis et al.
European journal of immunology, 21(9), 2087-2094 (1991-09-01)
The immune response to cholera toxin B subunit given orally was studied in 13 human volunteers. A serum IgG and IgA antitoxin response was observed, which was boosted by a second immunization. Using an immunospot assay, cells spontaneously secreting anti-toxin
Development of a Surface Plasmon Resonance?Based
Immunoassay for Listeria monocytogenes
Leonard P, et al.
Journal of Food Protection, 68(4), 728-735 null
Viswanath Arutla et al.
ACS combinatorial science, 19(5), 286-298 (2017-04-07)
Since the demonstration of nicotine vaccines as a possible therapeutic intervention for the effects of tobacco smoke, extensive effort has been made to enhance nicotine specific immunity. Linker modifications of nicotine haptens have been a focal point for improving the
G Kijanka et al.
Gut, 59(1), 69-78 (2009-10-16)
Patients with cancer have antibodies against tumour antigens. Characterising the antibody repertoire may provide insights into aberrant cellular mechanisms in cancer development, ultimately leading to novel diagnostic or therapeutic targets. The aim of this study was to characterise the antibody
Edward W Dervan et al.
Investigative ophthalmology & visual science, 51(6), 2968-2975 (2010-01-29)
Complex repertoires of IgG autoantibodies have been detected against ocular antigens in patients with glaucoma. The goal was to identify and characterize the IgG autoantibody repertoires in sera of patients with pseudoexfoliation glaucoma (PXFG) with protein macroarrays. Serum samples of

文章

The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.

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