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重要文件

A9316

Sigma-Aldrich

抗-小鼠 IgG(全分子)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

同義詞:

Goat Anti-Mouse IgG (whole molecule)−AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

技術

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

Goat polyclonal anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody is reactive with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Cross reactivity of the antibody conjugate is determined by ELISA. The conjugate shows no reactivity with rat IgG or rat serum proteins.
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant immunoglobulin present in human serum. The four classes of IgG are IgG1, IgG2, IgG3, and IgG4.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

應用

在含有终浓度为0.5M NaCl的TBS/Tween中,使用碱性磷酸酶缀合的山羊抗小鼠IgG(μg/ ml)作为二抗,对支气管肺泡液中的表面活性蛋白A进行检测。
Anti-Mouse IgG (whole molecule) Alkaline Phosphatase antibody produced in goat has been used as a secondary antibody in:
  • histological studies in heart and liver grafts
  • immunohistochemical studies of gangliosides and immunostaining of lymphoid organs
  • western blot detection in blood samples
The level of antibodies specific to P. aeuginosa present in immunized mouse sera was determined by ELISA using alkaline phosphatase-conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:1000.

生化/生理作用

IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections. IgG3 mediates effector functions and IgG4 is associated with asymptomatic infection.

其他說明

Antibody adsorbed with rat serum proteins.

外觀

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

準備報告

Adsorbed to reduce background with rat samples.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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T Saiki et al.
Transplantation, 71(12), 1806-1815 (2001-07-17)
Kinetics and role of host and donor dendritic cells (DCs) in transplantation immunity are still ill-defined. Using a rat cardiac transplantation model, we studied DC trafficking and sites for allosensitization. Host and donor DCs were defined as host- or donor-type
Alcohol administration blocks stress-induced impairments in memory and anxiety, and alters hippocampal neurotransmitter receptor expression in male rats
Gomez JL, et al.
Hormones and Behavior, 63(4), 659-666 (2013)
Simultaneous immunohistochemical detection of gangliosides and neuronal markers in paraformaldehyde-fixed nervous tissues by acetone etching
Sakumoto Y, et al.
Archives of Histology and Cytology, 72(2), 77-90 (2009)
Masafumi Ito et al.
Journal of neurology, neurosurgery, and psychiatry, 82(3), 294-299 (2011-01-22)
Ataxic Guillain-Barré syndrome is characterised by profound ataxia with negative Romberg sign and no ophthalmoplegia. Its nosological relationship to acute sensory ataxic neuropathy has yet to be discussed. Medical records were reviewed of patients suffering acute ataxia and reduced muscle
The microstructure of secondary lymphoid organs that support immune cell trafficking
Matsuno K, et al.
Archives of Histology and Cytology, 73(1), 1-21 (2010)

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