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Merck
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重要文件

90182

Sigma-Aldrich

Nα-甲苯磺酰基- L -赖氨酸氯甲基酮 盐酸盐

≥99.0% (AT)

同義詞:

(3S)-1-氯-3-甲苯磺酰氨基-7-氨基-2-庚酮 盐酸盐, (3S)-7-氨基-1-氯-3-甲苯磺酰氨基-2-庚酮 盐酸盐, TLCK, 甲苯磺酰基- L -赖氨酰-氯甲烷 盐酸盐

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About This Item

經驗公式(希爾表示法):
C14H21ClN2O3S · HCl
CAS號碼:
分子量::
369.31
Beilstein:
7106867
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
eCl@ss:
32160406
PubChem物質ID:
NACRES:
NA.77

生物源

synthetic

品質等級

化驗

≥99.0% (AT)

形狀

powder or crystals

光學活性

[α]20/D −7.8±0.5°, c = 2% in H2O

儲存溫度

−20°C

SMILES 字串

Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl

InChI

1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1

InChI 密鑰

YFCUZWYIPBUQBD-ZOWNYOTGSA-N

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其他說明

一种可能与酶活性位点上的组氨酸或半胱氨酸残基反应的烷基化试剂。灭活丝氨酸蛋白酶,如胰蛋白酶 和梭菌蛋白酶 。糜蛋白酶不受影响。为了防止蛋白水解降解,TLCK 和 TPCK 的组合可用于整个蛋白的分离过程,如分离组蛋白。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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分析證明 (COA)

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Further observations on substrate-derived chloromethyl ketones that inactivate trypsin.
E Shaw et al.
Archives of biochemistry and biophysics, 139(2), 298-305 (1970-08-01)
J M Griscavage et al.
Biochemical and biophysical research communications, 215(2), 721-729 (1995-10-13)
The objective of this study was to ascertain the mechanism by which serine and cysteine proteinase inhibitors interfere with production of NO by LPS-activated rat alveolar macrophages. Macrophages were incubated in the presence of LPS+ test agent for 24 hr.
Studies on the active site of clostripain. The specific inactivation by the chloromethyl ketone derived from -N-tosyl-L-lysine.
W H Porter et al.
The Journal of biological chemistry, 246(24), 7675-7682 (1971-12-25)
M K Urban et al.
Biochemistry, 18(18), 3952-3960 (1979-09-04)
Chicken erythrocyte histones 2A, 2B, and 3 can be resolved into nonallelic primary structure variants by polyacrylamide gel electrophoresis in the presence of Triton X-100. These variants were isolated and characterized by analysis of their tryptic and thermolytic peptides. The
I Kourteva et al.
Analytical biochemistry, 162(2), 345-349 (1987-05-01)
A technique for quickly detecting nanogram quantities of low- and high-molecular-weight inhibitors of some serine proteases is described. The inhibitor solutions are spotted onto agar films which contain either L-1-p-tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-trypsin or tosyl lysine chloromethyl ketone (TLCK)-chymotrypsin. Enzyme

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