616382

N^α-Tosyl-Lys Chloromethyl Ketone, Hydrochloride

Inhibits trypsin-like serine proteinases.

• 同義詞: N^α-Tosyl-Lys Chloromethyl Ketone, Hydrochloride, TLCK
• 經驗公式（希爾表示法）: C₁₄H₂₁ClN₂O₃S · xHCl
• CAS號碼: 4238-41-9
• 分子量：: 332.85 (free base basis)
• MDL號碼: MFCD00065395
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 品質等級: 100
• 描述: RTECS - XT5160000
• 化驗: ≥95% (elemental analysis)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic)
• 顏色: off-white
• 溶解度: aqueous solution, pH <6.0: 1 mg/mL
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• SMILES 字串: [S](=O)(=O)(N[C@@H](CCCC[N+H3])C(=O)CCl)c1ccc(cc1)C.[Cl-]
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
• InChI 密鑰: YFCUZWYIPBUQBD-ZOWNYOTGSA-N

描述

一般說明

Inhibits trypsin-like serine proteases. Inactivates trypsin irreversibly without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC₅₀ = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Suggesting working concentration is 10-100 µM.

Inhibits trypsin-like serine proteinases. Irreversibly inactivates trypsin without affecting chymotrypsin. Prevents nitric oxide production by activated macrophages by interfering with transcription of the iNOS gene. Blocks cell-cell adhesion and binding of HIV-1 virus to the target cells. In macrophages, blocks nitric oxide synthase induced by interferon-γ and lipopolysaccharides (EC₅₀ = 80 µM). Prevents endonucleolysis accompanying apoptotic death of HL-60 leukemia cells and normal thymocytes.

生化／生理作用

Cell permeable: no

EC50 = 80 µM blocking nitric oxide synthase induced by interferon-γ and lipopolysaccharides in macrophages

Primary Target
trypsin like proteases

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Irritant (B)

重構

Unstable in solution; reconstitute just prior to use.

其他說明

Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Kim, H., et al. 1995. J. Immunol.154, 4741.
Bourinbaiar, A.S. and Nagorny, R. 1994. Cell Immunol. 155, 230.
Biro, A., et al. 1992. Eur. J. Immunol.22, 2547.
Bruno, S., et al. 1992. Leukemia6, 1113.
Lee, S.F. 1992. Infect. Immun. 60, 4032.
Schmidt, H.H., et al. 1992. Mol. Pharmacol.41, 615.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable