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Key Documents

T7902

Sigma-Aldrich

嗜热菌蛋白酶 from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

同義詞:

嗜热菌蛋白酶, 蛋白酶 来源于六甲嗜热溶蛋白芽孢杆菌

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.75

生物源

Geobacillus stearothermophilus

品質等級

產品線

BioReagent

形狀

powder

比活性

30-350 units/mg protein (E1%/280)

分子量

34.6 kDa

純化經由

crystallization

技術

cell culture | mammalian: suitable
single cell analysis: suitable

儲存溫度

−20°C

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相關類別

應用

一种含有四个钙离子的热稳定性(嗜热性)细胞外金属内肽酶。辅助因子是锌和钙。水解疏水性氨基酸残基N-末端的蛋白质键。最适pH为8.0,活性的最适温度为70℃。在pH 5-9.5范围内非常稳定。嗜热菌蛋白酶具有较低的切割特异性,因此,它能产生许多适合测序的短片段。优先切割顺序: X-切割-Y-Z,其中X = 任何氨基酸;Y = Leu,Phe,Ile,Val,Met,Ala;Z是除Pro以外的任何氨基酸。切割Leu的N末端优先于切割Phe的N末端,它们优于其他氨基酸。常用于限制性蛋白水解以绘制肽图谱以及蛋白质结构和构象变化研究。

單位定義

在37℃、pH7.5下,一个单位每分钟将水解酪蛋白产生相当于1.0 μmol (181 μg)7.5克酪氨酸(福林-乔卡尔特试剂显色)。

外觀

含钙和乙酸钠缓冲盐的冻干粉

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析證明 (COA)

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