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Merck
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重要文件

53702

Millipore

Pronase®蛋白酶,灰色链霉菌

同義詞:

Pronase® powder

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.54

描述

RTECS - UK9595000

品質等級

形狀

lyophilized

比活性

≥45,000 proteolytic units/g dry wt

製造商/商標名

Calbiochem®

儲存條件

OK to freeze
desiccated (hygroscopic)

溶解度

water: 10 mg/mL

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

一种非特异性蛋白酶将粘蛋白液化并消化蛋白质以释放氨基酸。可用于分离活的软骨细胞。在中性pH下最具活性,但可在较宽的pH和温度范围内稳定。最适pH为7.5。注意:1 KU = 1000单位。
注意:1 KU = 1000单位。

警告

毒性:有害(C)

單位定義

一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。

外觀

从含有乙酸钙作为稳定剂的溶液进行冻干。

重構

在重悬后存放于冰箱(4°C)。储备液可在4°C稳定24小时。

其他說明

Narahashi, Y., et al. 1968.J. Biochem.64, 427.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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W N Ross et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 4(3), 659-672 (1984-03-01)
Optical techniques using voltage-sensitive dyes were used to record electrical events simultaneously from many positions on certain neurons of the barnacle supraesophageal ganglion. By signal-averaging, recordings with good signal-to-noise ratios and time resolution were obtained from fine dendritic processes as
T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
Saurabh Dahiya et al.
Human molecular genetics, 20(22), 4345-4359 (2011-08-19)
Matrix metalloproteinases (MMPs) are a group of extracellular proteases involved in tissue remodeling in several physiological and pathophysiological conditions. While increased expression of MMPs (especially MMP-9) has been observed in skeletal muscle in numerous conditions, their physiological significance remains less-well
M Tsacopoulos et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 17(7), 2383-2390 (1997-04-01)
Glial cells transform glucose to a fuel substrate taken up and used by neurons. In the honeybee retina, photoreceptor neurons consume both alanine supplied by glial cells and exogenous proline. Ammonium (NH4+) and glutamate, produced and released in a stimulus-dependent
S Kästner et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(8), 1079-1096 (2000-07-18)
Satellite cells are the myogenic precursors in postnatal muscle and are situated beneath the myofiber basement membrane. We previously showed that fibroblast growth factor 2 (FGF2, basic FGF) stimulates a greater number of satellite cells to enter the cell cycle

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