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一般說明
蛋白水解酶具有催化、非催化和辅助结构域。蛋白水解酶大致分为内肽酶和外肽酶。根据功能可分为天冬氨酸、谷氨酸、半胱氨酸、苏氨酸、丝氨酸和金属蛋白酶。
應用
Sigma 公司的酶已被用于制备鸡(腿)骨蛋白水解产物,同时评估 ACE 抑制肽。在研究它们的抗肿瘤活性时,它还被用于水解蛋白核小球藻(Chlorella pyrenoidosa)蛋白质提取物。
蛋白酶是一种通过水解肽键来分解蛋白的酶。 蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。
生化/生理作用
碱性蛋白酶是丝氨酸型的内切蛋白酶。它具有广泛的底物特异性,可以水解蛋白质分子中的大多数肽键。它在 pH 6.5 和 8.5 之间有活性,最适温度为 60°C。该酶可用在洗涤剂配方中,以去除蛋白质污渍。
蛋白酶通过肽键的水解使蛋白质分解代谢。 蛋白酶被丝氨酸活性位点抑制剂灭活,例如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。
分析報告
用安森单位表示的活性
法律資訊
Novozyme公司的产品
Alcalase is a registered trademark of Novozymes Corp.
訊號詞
Danger
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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