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種類
Type XXIV
形狀
lyophilized powder
比活性
≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid
分子量
27 kDa
純化經由
crystallization
運輸包裝
wet ice
儲存溫度
−20°C
特異性
枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白，并在碱性条件下具有活性。
應用
来自sigma的该酶已用于优化大鼠心脏均质化心室组织中所有线粒体群的释放。它还用于福尔马林固定石蜡包埋肝脏样本的预杂交处理，用于人和病毒DNA的检测。
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。 产品P8038，也被称为枯草杆菌蛋白酶Carlsberg，已用于水解心肌细胞以研究心脏线粒体NHE1的沉默。
生化／生理作用
蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。
物理性質
枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。
單位定義
在pH 7.5，37 °C 条件下，一单位的酶每分钟将会水解酪蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。
分析報告
氨基酸分析和等电聚焦电泳与枯草杆菌蛋白酶Carlsberg一致。
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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实验方案
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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