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Merck

P4032

Sigma-Aldrich

朊酶 来源于蜂蜜曲霉

Type XXIII, ≥3 units/mg solid

别名:

Protease from Aspergillus melleus, Proteinase from Aspergillus sp.

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

生物源

Aspergillus sp. (A. melleus)

種類

Type XXIII

形狀

solid

比活性

≥3 units/mg solid

儲存溫度

2-8°C

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相关类别

應用

蛋白酶是一种通过水解肽键来分解蛋白质的酶。蛋白酶用于降解蛋白质、研究蛋白酶抑制剂和研究热灭活动力学。蛋白酶用于孵育过程中的核酸分离程序。它被用于研究蛋白酶激活受体,如炎症和免疫反应中蛋白酶介导的信号转导 。产品 P4032 来自 黑曲霉 ,已用于霍氏链霉菌木聚糖酶的非特异性降解。

生化/生理作用

蛋白酶通过水解肽键使蛋白质分解代谢。丝氨酸活性部位抑制剂可灭活蛋白酶,如苯甲基磺酰氟 (PMSF) 和二异丙基氟磷酸盐。

單位定義

除非另有说明,否则一个单位将水解酪蛋白以在 pH7.5,37°C 下每分钟产生相当于1.0μmole (181μg) 酪氨酸的颜色(Folin-Ciocalteu 试剂的颜色)。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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实验方案

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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