一般說明
该酶在pH 8-10下稳定，可在24小时保存高达90%的活性。其55-60℃下具有最大活性。
特異性
枯草素A 是丝氨酸S8内蛋白酶家族的一个成员。它具有广泛的特异性，在P1位置有较大的非荷电残基。它可水解天然和变性蛋白，并且在碱性条件下具有活性。
應用
该产品已与其他酶一起用于 原位 蛋白水解，以制备适用于结构测定的晶体。它还被用于分离可用于 体外 功能研究的肌膜下(SS)和肌原纤维间(IMF)线粒体。
这是由 衣芽孢杆菌发酵而分离出来的一种蛋白水解酶。这是一种丝氨酸内蛋白酶，对天然和变性蛋白具有广泛的特异性，在碱性条件下具有活性。
生化／生理作用
蛋白酶通过水解肽键分解蛋白质。 蛋白酶可被丝氨酸活性位点抑制剂灭活，如苯基甲基磺酰氟(PMSF)和二异丙基氟磷酸酯。 蛋白酶，P5380的产物，是一种丝氨酸内蛋白酶，对天然和变性蛋白具有广泛的特异性，在碱性条件下具有活性。 它在某些有机溶剂中具有活性，如干辛烷。
物理性質
枯草素 是一种无二硫键的非糖基化单肽链，其分子量为27kda。
單位定義
一单位在37°C、pH 7.5条件下每分钟可水解1.0μmole(181 μg)的酪氨酸（福林酚试剂显色）。
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
Marie Deprez et al.
American journal of respiratory and critical care medicine, 202(12), 1636-1645 (2020-07-30)
Rationale: The respiratory tract constitutes an elaborate line of defense that is based on a unique cellular ecosystem.Objectives: We aimed to investigate cell population distributions and transcriptional changes along the airways by using single-cell RNA profiling.Methods: We have explored the
L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE
Christoph Schiklenk et al.
The Journal of cell biology, 217(7), 2383-2401 (2018-05-08)
Although the formation of rod-shaped chromosomes is vital for the correct segregation of eukaryotic genomes during cell divisions, the molecular mechanisms that control the chromosome condensation process have remained largely unknown. Here, we identify the C2H2 zinc-finger transcription factor Zas1
Michael L Mucenski et al.
Scientific reports, 9(1), 4557-4557 (2019-03-16)
The uterus is a remarkable organ that must guard against infections while maintaining the ability to support growth of a fetus without rejection. The Hoxa10 and Hoxa11 genes have previously been shown to play essential roles in uterus development and
Tommi Kotila et al.
Nature communications, 9(1), 1892-1892 (2018-05-16)
Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on "re-charging" of ADP-actin monomers with ATP, but whether this reaction requires dedicated proteins in cells, and the underlying mechanism, have remained elusive.
实验方案
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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