推荐产品
形狀
lyophilized powder
品質等級
品質
for use in Total Dietary Fiber Assay, TDF-100A
分子量
27 kDa
應用
food and beverages
運輸包裝
wet ice
儲存溫度
2-8°C
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特異性
枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白，并在碱性条件下具有活性。
應用
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。它用于总膳食纤维检测（TDF-100A）中。
生化／生理作用
蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。
物理性質
枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。
單位定義
在pH 7.5、37°C条件下，一个单位每分钟可水解酪蛋白，生成相当于1.0 μmole (181 μg)酪氨酸的颜色。
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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实验方案
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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