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產品線
BioReagent
形狀
powder
比活性
≥3.5 units/mg solid
技術
cell culture | embryo: suitable
雜質
starch, essentially free
儲存溫度
−20°C
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特異性
一种具有包括细胞外丝氨酸蛋白酶在内至少三种蛋白水解活性的混合物。通常，丝氨酸蛋白酶可显示出广泛的底物特异性，而这被视为通过分子中由一个Asp、一个His和Ser残基组成的活性位点所介导的。该酶更倾向于水解谷氨酸或天冬氨酸的羧基侧的肽键。
應用
蛋白酶通常用于核酸的分离过程，通过补充0.2％十二烷基硫酸钠和10 mM EDTA并孵育0.5-3.0小时。
该酶比已知碱性蛋白酶在更高的pH范围内更具活性，即使在0.2N NaOH溶液中也显示出了蛋白水解活性。该酶用于不溶性蛋白的蛋白水解和蛋白的结构研究。
其他說明
一种不常见的非特异性蛋白酶。
品質
含有约 25％ 的醋酸钙。
物理性質
通过在80 °C以上加热15-20分钟以完全灭活。
單位定義
在 pH7.5，37℃ 下，一个单元每分钟将水解酪蛋白，并产生相当于 1.0 μmole（181 μg）酪氨酸的颜色（使用 Folin-Ciocalteu 试剂显色）。
準備報告
收集自灰色链霉菌的培养基肉汤中。
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
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Shantha K Mahadevaiah et al.
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Single-cell RNA sequencing of embryos can resolve the transcriptional landscape of development at unprecedented resolution. To date, single-cell RNA-sequencing studies of mammalian embryos have focused exclusively on eutherian species. Analysis of mammalian outgroups has the potential to identify deeply conserved
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The gene (npr) encoding an extracellular neutral metalloprotease (Npr) from Streptomyces cacaoi YM15 was cloned in Streptomyces lividans using pIJ702 as a vector. The nucleotide (nt) sequence of npr was determined. The deduced open reading frame encoded 550 amino acids
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Human gene therapy, 30(2), 179-196 (2018-07-20)
Hepatic stellate cells (HSCs) are the primary cell type responsible for liver fibrogenesis. Transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor (PDGF) are key profibrotic cytokines that regulate HSC activation and proliferation with functional convergence. Dual RNA interference
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