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應用
细胞色素c氧化酶检测试剂盒使用了一种经过优化的比色检测方法,该方法基于550 nm处测得的高铁细胞色素c吸光度下降(这是由于高铁细胞色素c被细胞色素c氧化酶氧化造成的)进行检测。 该试剂盒适于检测线粒体外膜完整度/线粒体应激性,同时也适于检测亚细胞组分之中的线粒体。
生化/生理作用
细胞色素c氧化酶[EC 1.9.3.1]位于线粒体内膜上,将线粒体基质和膜间隙分离开来,一直被用作这种膜的标记物。在细菌的细胞质膜上,也存在细胞色素c氧化酶。细胞色素c氧化酶可通过氧化磷酸化过程连接细胞色素链之间的电子转运,为细胞提供能量。
特點和優勢
- 简单,经优化的实验方案 - 无需进行专门的培训,即可获得可重复的结果
- 可用于测定任意线粒体来源的细胞色素酶c的活性 - 这种酶在所有种类生物的线粒体中均存在
- 可用于检测亚细胞成分中是否存在线粒体 - 节省时间,同时提高了细胞器制备质量的可信度
- 可结合MITOISO1 Mitochondrial Isolation Kit使用- 标准化的线粒体制备和分析,确保了可重复性
- 可用于分析线粒体膜的完整性
- 简单的比色测定解决方案
相關產品
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
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