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CY0100

Sigma-Aldrich

细胞色素C还原酶(NADPH)检测试剂盒

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

200

用途

 kit sufficient for 100 tests

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... POR(5447)

相关类别

生化／生理作用

真核生物的NADPH-细胞色素C还原酶（NADPH-细胞色素P450还原酶，EC1.6.2.4）是一种位于内质网的黄素蛋白。它可将NADPH的电子传递到负责对异型生物质发挥解毒功能的多种氧合酶，其中最重要的是细胞色素P450家族的酶。NADPH-细胞色素C还原酶被广泛用作内质网的标记蛋白，以及生态污染和膳食脂质摄取的生物标记物。

特點和優勢

  • 用于使用密度梯度分离法在分离和亚细胞分离时检测内质网(ER)
  • 在药物中毒或外源性物质激发后，使用比色法检测多种来源的NADPH-细胞色素C还原酶
  • 用于分析内质网的聚集及其功能

分析報告

细胞色素C还原酶检测试剂盒可快速便捷地检测细胞、组织提取物以及纯化的微粒体中的NADPH-细胞色素C还原酶活性，其含有该检测所需的所有试剂。该试剂盒已通过对肝、肾、脑、脾和心肌等多种哺乳动物组织样本以及HeLa、HepG2和Jurkat等细胞裂解物的测试。此外，还通过了对P. pastoris 和 S. cerevisiae.酵母菌株样本进行的测试。

仅试剂盒组分

产品编号
说明
  • Assay Buffer 100 mL
  • Enzyme Dilution Buffer 25 mL
  • Cytochrome c 50 mg
  • Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
  • Cytochrome c Oxidase Inhibitor Solution 1 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg化学品安全说明书

相關產品

产品编号
说明
价格

象形圖

Skull and crossbones
GHS06

訊號詞

Danger

危險聲明

H301,H412

危險分類

Acute Tox. 3 Oral - Aquatic Chronic 3

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
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In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The
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