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Sigma-Aldrich

线粒体分离试剂盒

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

别名:

线粒体提取试剂盒, 线粒体纯化试剂盒

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

品質等級

200

用途

sufficient for 50 applications (2-5 x 107 cells)

技術

ELISA: suitable
western blot: suitable

運輸包裝

dry ice

儲存溫度

−20°C

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相关类别

一般說明

线粒体是一种双层膜结构，是真核细胞中大部分能量产生的位点。线粒体分离试剂盒包含两种缓冲液：一种用于提取功能研究的蛋白质，另一种用于蛋白质组学研究的分析。此外，该试剂盒还包括保存完整线粒体的存储缓冲液。

應用

线粒体分离试剂盒用于分离下列来源中的线粒体：
  • Ewing′肉瘤肿瘤家族(ESFT)细胞
  • 肺结核患者血液样本
  • 人神经母细胞瘤细胞和神经胶质瘤细胞
  • 肌小管
  • HepG2细胞

特點和優勢

  • 该试剂盒允许快速和简单地从细胞中提取浓缩的线粒体部分。
  • 分离的线粒体大多具有完整的内外膜。
  • 可用于提取线粒体蛋白进行蛋白质组分析

其他說明

  • 本产品仅供研发使用，不用于药物、家庭或其他用途。
  • 请查阅材料安全数据表，了解有关危险和安全处理方法的信息。
  • 使用超纯水制备试剂。
  • 请参阅产品信息表以了解有关试剂盒成分、所需试剂、存储和稳定性以及隔离方案的更多信息。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL化学品安全说明书
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL化学品安全说明书
  • C0356Protein Extraction Reagent Type 4 1 bottle化学品安全说明书

訊號詞

Warning

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

閃點（°F）

188.6 °F - closed cup

閃點（°C）

87 °C - closed cup

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
019M4159V
11180549
048M4101V
11171133
097M4114V

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Down syndrome (trisomy of human chromosome 21) is a common genetic disorder. Overproduction of the gaseous mediator hydrogen sulfide (H2S) has been implicated in the pathogenesis of neurological and metabolic deficits associated with Down syndrome. Several lines of data indicate
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The Journal of biological chemistry, 278(2), 1346-1353 (2002-10-31)
We have shown here that the apoptosis inducer staurosporine causes an early decrease in the endogenous respiration rate in intact 143B.TK(-) cells. On the other hand, the activity of cytochrome c oxidase is unchanged for the first 8 h after
The carbonic anhydrase inhibitor methazolamide prevents amyloid beta-induced mitochondrial dysfunction and caspase activation protecting neuronal and glial cells in vitro and in the mouse brain
Fossati S, et al.
Neurobiology of Disease, 86, 29-40 (2016)
D W Moss et al.
The European journal of neuroscience, 13(3), 529-538 (2001-02-13)
Activation of murine microglial and macrophage cell lines with lipopolysaccharide (LPS) and interferon-gamma (IFN-gamma) resulted in the induction of the inducible form of nitric oxide synthase (NOS) and the release of micromolar amounts of NO into the surrounding medium. The
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At present, there is no efficient curative therapy for cancer patients with advanced metastatic disease. Targeting of antiapoptotic molecules acting on the mitochondrial apoptosis pathway could potentially augment antimetastatic effect of cytotoxic drugs. Similarly to Bcl-2 family members, beta-galactoside-binding lectin
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