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檢測方法
colorimetric
相關疾病
endocrinological disorders, diabetes; cancer
儲存溫度
−20°C
基因資訊
human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)
一般說明
柠檬酸合酶（CS；EC 2.3.3.1）是一种线粒体酶，存在于所有能够进行氧化代谢的细胞中。它在Krebs 三羧酸循环中，催化草酰乙酸合成柠檬酸盐。1,2柠檬酸合酶参与能量产生、脂肪生成和胆固醇生成，其活性遵循昼夜节律模式。
應用
柠檬酸合酶活性检测试剂盒已用于测量样品中柠檬酸合酶的活性。
適合性
适于测定不同生物样品内的柠檬酸合成酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原則
柠檬酸合酶活性检测试剂盒提供了一种简单直接的方法，用于测量各种组织、细胞培养物（粘附或悬浮）和纯化线粒体中柠檬酸合酶的活性。使用偶联酶反应测定柠檬酸合酶活性，其产生与存在的酶活性成比例的比色（412 nm）产物。一个单位的柠檬酸合酶是在25 °C和pH 7.2 下，每分钟产生 1.0 mmole CoA的酶的量。
仅试剂盒组分
产品编号
说明
- CS Assay Buffer
- CS Substrate Mix
- CS Developer
- GSH Standard, Reduced
- CS Positive Control
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
Diogo T Galan et al.
American journal of physiology. Heart and circulatory physiology, 311(5), H1075-H1090 (2016-11-03)
Scarring and remodeling of the left ventricle (LV) after myocardial infarction (MI) results in ischemic cardiomyopathy with reduced contractile function. Regional differences related to persisting ischemia may exist. We investigated the hypothesis that mitochondrial function and structure is altered in
Tomoaki Aoki et al.
Frontiers in medicine, 8, 666735-666735 (2021-04-30)
Background: Identification of the mechanisms underlying mitochondrial dysfunction is key to understanding the pathophysiology of acute injuries such as cardiac arrest (CA); however, effective methods for measurement of mitochondrial function associated with mitochondrial isolation have been debated for a long
Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte
Md Habibur Rahman et al.
Microsystems & nanoengineering, 4, 39-39 (2019-05-07)
Defective mitochondria have been linked to several critical human diseases such as neurodegenerative disorders, cancers and cardiovascular disease. However, the detailed characterization of mitochondria has remained relatively unexplored, largely due to the lack of effective extraction methods that may sufficiently
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