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檢測方法
colorimetric
相關疾病
neurological disorders
儲存溫度
−20°C
基因資訊
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
一般說明
苹果酸脱氢酶（MDH; EC 1.1.1.37）是一种NAD氧化还原酶，其可催化苹果酸盐和草酸乙酯的NAD/NADH依赖性相互转化。该反应对于维持苹果酸/天冬氨酸穿梭线粒体膜和线粒体基质中的Kerbs三羧酸循环来说至关重要。研究人员已在诸如阿尔茨海默氏病′的神经变性疾病中观察到MDH活性的增加。
應用
苹果酸脱氢酶测定试剂盒已被用于LDH（乳酸脱氢酶）和苹果酸脱氢酶（MDH1）的测定。 它也被用于估测异柠檬酸脱氢酶 和琥珀酸脱氢酶的活性。
適合性
适于测定不同生物样品内的苹果酸脱氢酶活性，如细胞和组织培养上清液以及纯化所得的线粒体。
原則
苹果酸脱氢酶检测试剂盒提供了一种简单灵敏的方法，用于测定各种组织、细胞培养物和分离的线粒体中的MDH活性。通过产生与酶活性成比例的在450nm处有吸收的产物来确定MDH的活性。在37℃，pH 9.5条件下，一个单位MDH为每分钟产生1.0 μmol NADH所需的酶量。
仅试剂盒组分
产品编号
说明
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
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