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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
cancer
儲存溫度
−20°C
基因資訊
human ... IDH1(3417) , IDH2(3418) , IDH3B(3420) , IDH3G(3421)
mouse ... IDH1(15926) , IDH2(269951) , IDH3B(170718) , IDH3G(15929)
rat ... IDH1(24479) , IDH2(361596) , IDH3B(94173) , IDH3G(25179)
一般說明
异柠檬酸脱氢酶 (IDH) 催化异柠檬酸转化为 α-酮戊二酸。在真核生物中,IDH 有三种同工酶,线粒体 IDH2 和 IDH3 以及细胞质/过氧化物酶体 IDH1。所有三个 IDH 家族成员的酶活性均需要存在二价阳离子(Mg 2 + 或 Mn 2 + )和电子接受辅助因子 NADP + (IDH1 和 IDH2)或 NAD + (IDH3)。在某些癌症如胶质母细胞瘤、急性髓性白血病和结肠癌中发现 IDH1 和 IDH2 突变导致新形成层酶活性。该新活性显示底物特异性发生变化,导致 α-酮戊二酸转化为 2-羟基戊二酸。IDH 家族成员的突变也与 Ollier 病和 Maffucci 综合征有关。
應用
异柠檬酸脱氢酶 (IDH) 活性测定试剂盒用于测定样品中异柠檬酸脱氢酶的活性。
特點和優勢
与高通量处理系统兼容。
適合性
适用于测定生物样本(包括组织、细胞和血清)中的异柠檬酸脱氢酶 (IDH)(NAD +-依赖性、NADP + 依赖性或两种 IDH)活性
原則
异柠檬酸脱氢酶活性测定试剂盒为测定多种样品中 NADP + 依赖性、NAD + 依赖性或 NADP + 和 NAD + 依赖性 IDH 活性提供了一种简单直接的方法。在酶反应中使用异柠檬酸盐作为底物测定 IDH 活性,得到比色产物 (450 nm) 与存在的酶活性成正比。一个单位的 IDH 是在 37°C、pH 8.0 时每分钟生成 1.0 μmol NADH 或 NADP 的酶量。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
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实验方案
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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