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C2506

Sigma-Aldrich

细胞色素 C 来源于马心脏

≥95% (SDS-PAGE)

别名:

Cytochrome c 来源于马心脏

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About This Item

CAS号:
9007-43-6
EC號碼:
232-700-9
MDL號碼:
MFCD00130890
分類程式碼代碼:
12352202
NACRES:
NA.61

生物源

horse heart

品質等級

300

化驗

≥95% (SDS-PAGE)

形狀

powder

分子量

12,384

技術

cell based assay: suitable

溶解度

H2O: soluble 10 mg/mL

適合性

suitable for molecular biology

UniProt登錄號

P00004

應用

cell analysis

儲存溫度

−20°C

基因資訊

horse ... CYCS(100053958)

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應用

细胞色素c已用于：
  • 还原由黄嘌呤氧化酶生成的超氧自由基-铁氧化铬c
  • 采用MALDI质谱法，用单液滴沉积法进行分析
  • 作为标准细胞色素c还原酶活性测定的末端电子受体
  • 细胞色素c氧化酶组织化学
  • 用于细胞色素C氧化酶活性的测定
  • 作为磷酸盐缓冲液的组分，用于采用蓝色天然凝胶电泳 (BN-PAGE) 检测和评估复合物 IV 活性
  • 用于检测离体肾脏吞噬细胞中的细胞外超氧阴离子 (ECSA)
马的细胞色素c经过H2O2和UV 处理后的特异性位点和氧化程度已经过表征。研究表明这些位点可能可用作细胞内氧化环境的生物标记。

生化／生理作用

细胞色素 c 从线粒体外层释放到胞质溶胶中，有助于激活 caspase-9 并启动凋亡蛋白酶级联反应。其与凋亡蛋白酶激活因子-1 (APAF-1) 和三磷酸脱氧腺苷 (dATP) 的相互作用可促进这一过程。(3)各种研究表明，测定肿瘤患者血清中的细胞色素 C 水平可以被认为是体内细胞死亡的潜在标志。
细胞色素c的主要身份是电子载体线粒体蛋白。在细胞内，细胞色素c能够在二价铁和三价铁之间转化，使得它称为了有效的生物电子载体，在动植物细胞内氧化中起到很重要的作用。它通常被视作呼吸作用催化剂，在呼吸底物和氧气之间形成必需的电子桥

準備報告

Prepared using TCA.
This product in solution should be dark red to reddish brown when dissolved in water at 10 mg/mL.

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
想要了解更多细胞色素c和电子传递的信息，请访问www.sigma-aldrich.com/enzymeexplorer.

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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B C Hill
The Journal of biological chemistry, 266(4), 2219-2226 (1991-02-05)
The reaction of the electrostatic cytochrome c-cytochrome oxidase complex with oxygen is measured by transient absorption spectroscopy. The oxygen reaction is initiated by photolytic removal of CO from cytochrome oxidase, using a flash-pumped dye laser. The subsequent reaction of the
Vittorio Pandini et al.
The Journal of biological chemistry, 277(50), 48463-48471 (2002-10-09)
Toxoplasma gondii possesses an apicoplast-localized, plant-type ferredoxin-NADP(+) reductase. We have cloned a [2Fe-2S] ferredoxin from the same parasite to investigate the interplay of the two redox proteins. A detailed characterization of the two purified recombinant proteins, particularly as to their
Enzyme-catalyzed free radical reactions with nicotinamide adenine nucleotides. II. Lactate dehydrogenase-catalyzed oxidation of reduced nicotinamide adenine dinucleotide by superoxide radicals generated by xanthine oxidase.
P C Chan et al.
The Journal of biological chemistry, 249(4), 1317-1319 (1974-02-25)
Attila I Gulyás et al.
The European journal of neuroscience, 23(10), 2581-2594 (2006-07-05)
Cytochrome c (CC) immunoreactivity was quantified in functionally distinct rat hippocampal inhibitory neuron populations using double immunocytochemistry and laser scanning confocal microscopy to measure the CC expression level as well as the amount of mitochondria within the cells, which is
Ian M Morison et al.
Nature genetics, 40(4), 387-389 (2008-03-18)
We report the first identified mutation in the gene encoding human cytochrome c (CYCS). Glycine 41, invariant throughout eukaryotes, is substituted by serine in a family with autosomal dominant thrombocytopenia caused by dysregulated platelet formation. The mutation yields a cytochrome
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HPLC Analysis of Proteins on TSKgel® BioAssist S

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Electron Transport

Learn about the four membrane-bound protein complexes that make up the electron transport chain metabolic pathway supplying energy as ATP for cellular respiration.

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

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Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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