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B6185

Sigma-Aldrich

Bionic™ Buffer, 10X Concentrate

for Northern and Southern blotting, solution

别名:

Sodium Borate Electrophoresis Buffer

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

等級

for molecular biology

品質等級

200

描述

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

形狀

solution

濃度

10 ×

雜質

DNase, RNase and nickase, none detected

pH值

8.0-8.5 (20-25 °C)

儲存溫度

room temp

相关类别

一般說明

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

應用

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

特點和優勢

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

包裝

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

注意

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

法律資訊

Bionic is a trademark of Sigma-Aldrich Co. LLC

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險聲明

H319,H360FD

危險分類

Eye Irrit. 2 - Repr. 1B

儲存類別代碼

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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实验方案

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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