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H7033

Sigma-Aldrich

PerfectHyb™ Plus杂交缓冲液

for Northern and Southern blotting, solution

别名:

hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

一般說明

Sigma的PerfectHyb Plus是一种即用型杂交缓冲液，适用于Northern和Southern印迹方案。 使用PerfectHyb Plus可将杂交时间缩短至两小时。

應用

可用作Northern印迹、Southern印迹、菌落/噬斑印迹和斑点印迹中所使用的杂交缓冲液。

特點和優勢

  • 快速且易于使用
  • 灵敏度比传统缓冲液高20倍
  • 不需要封闭步骤
  • 与中性和带正电的膜兼容
  • 与化学发光、比色和DIG检测分析兼容
  • 兼容放射性和非放射性探针
  • 与单链或双链DNA、RNA和寡核苷酸探针兼容

其他說明

在溶液中形成的少量沉淀物可以在30-35℃下，通过偶尔轻缓颠倒进行溶解。

法律資訊

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
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miRNAs are known to play important regulatory roles throughout plant development. Until recently, nearly all the miRNAs in maize were identified by comparative analysis to miRNAs sequences of other plant species, such as rice and Arabidopsis. To find new miRNA
Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
Angel Emilio Martínez de Alba et al.
Nucleic acids research, 39(21), 9339-9344 (2011-08-05)
In plants, most microRNAs (miRNAs) and several endogenous small interfering RNAs (siRNAs) bind to ARGONAUTE1 (AGO1) to regulate the expression of endogenous genes through post-transcriptional gene silencing (PTGS). AGO1 also participates in a siRNA-mediated PTGS defense response that thwarts exogenous
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PloS one, 6(11), e27736-e27736 (2011-11-24)
The potato/tomato psyllid, Bactericerca cockerelli (B. cockerelli), and the Asian citrus psyllid, Diaphorina citri (D. citri), are very important plant pests, but they are also vectors of phloem-limited bacteria that are associated with two devastating plant diseases. B. cockerelli is
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实验方案

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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