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Key Documents

S6639

Sigma-Aldrich

SSC 20x 浓缩缓冲液

for Northern and Southern blotting, solution

别名:

20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

等級

for molecular biology

品質等級

200

描述

Saline-Sodium Citrate buffer, made with ultrapure water

無菌

0.2 μm filtered

形狀

solution

應用

diagnostic assay manufacturing

異物活動

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

儲存溫度

room temp

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相关类别

一般說明

SSC（柠檬酸钠盐）缓冲液20倍浓缩液是Southern和Northern杂交过程的标准试剂。 它可用于严格控制杂交后洗涤步骤所用洗涤缓冲液。

應用

SSC缓冲液20倍浓缩液已用于
  • 用作原位杂交人特异性重复Alu序列的杂交缓冲液
  • 用作Southern杂交、小鼠基因组DNA斑点印迹和microRNA的Northern印迹的印迹膜洗涤缓冲液
  • 用作L1反转录转座子荧光原位杂交(FISH)的孵育缓冲液

特點和優勢

  • 方便的即用型液体
  • 在杂交中具有多种用途

成分

SSC缓冲液20倍浓缩液由3M氯化钠、0.3M柠檬酸钠组成（pH7.0）。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
Chang-Gong Liu et al.
Methods (San Diego, Calif.), 44(1), 22-30 (2007-12-26)
After 12 years from its first application, microarray technology has become the reference technique to monitor gene expression of thousands of genes in the same experiment. In the past few years an increasing amount of evidence showed the importance of
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
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Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

实验方案

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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