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Merck

C7594

Sigma-Aldrich

酪蛋白封闭缓冲液

for Northern and Southern blotting, powder blend

别名:

blocking buffer

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About This Item

EC號碼:
分類程式碼代碼:
12161700
NACRES:
NA.25

等級

for molecular biology

品質等級

形狀

powder blend

技術

Southern blotting: suitable

pH值

6.8-7.2(reconstituted per directions)

溶解度

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

儲存溫度

room temp

相关类别

一般說明

酪蛋白是一种牛奶来源的物质,通常用于 Western、Southern 和 Northern 印迹实验中的封闭缓冲液。封闭剂用于减少检测试剂与膜的非特异性结合。

應用

适合用作 Western、Southern 和 Northern 印迹膜的封闭缓冲液。然后,封闭膜可用于化学发光,比色或 DIG 检测分析。

特點和優勢

  • 封闭性能优于奶粉或 BSA
  • 与中性和正电荷膜兼容
  • 与化学发光,比色和 DIG 检测分析兼容

準備報告

将瓶中的全部内容物溶解在 800 mL 分子生物学级水中。搅拌直至充分混合,然后加水至终体积 1L。在 2-8°C 下最多保存一周。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


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Tadatsura Koshika et al.
Immunology, 134(4), 386-397 (2011-11-03)
α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering
W Querbes et al.
Journal of virology, 80(19), 9402-9413 (2006-09-16)
Invasion of glial cells by the human polyomavirus, JC virus (JCV), leads to a rapidly progressing and uniformly fatal demyelinating disease known as progressive multifocal leukoencephalopathy. The endocytic trafficking steps used by JCV to invade cells and initiate infection are
Seiichi Katayama et al.
Acta medica Okayama, 60(6), 351-355 (2006-12-27)
Clostridium perfringens is an anaerobic spore-forming pathogen of humans and animals. C. perfringens type A strains, 13, CPN50, and NCTC8237, isolated from human gas gangrene, bound specifically to human fi bronectin (Fn). The trypsin-treatment of the bacterial cells significantly reduced
Xiaoli Ma et al.
BMC biochemistry, 11, 37-37 (2010-09-24)
Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
Radoi, A., et al.
Analytical Letters, 42, 1187-1187 (2009)

实验方案

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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