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Merck
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主要文件

P1415

Sigma-Aldrich

预杂交溶液

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

价格与库存信息目前不能提供

品質等級

100
200

形狀

liquid (2x concentrate)

異物活動

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

儲存溫度

−20°C

一般說明

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液,适用于Northern和Southern印迹方案。

應用

适合用作Northern和Southern印迹方案的预杂交缓冲液。

成分

Sigma的2倍预杂交液含200 μg/mL DNA,溶剂为10倍SSC和2倍Denhardt溶液:
  • 200 μg/mL DNA
  • 2mg牛血清白蛋白(BSA)
  • 2mg聚蔗糖
  • 2mg聚乙烯吡咯烷酮(PVP)
  • 0.15 M柠檬酸钠
  • 1.5M NaCl

相關產品

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

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    实验方案

    An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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